https://doi.org/10.4081/bse.105
In vitro microfluidic modelling of the human blood-brain-barrier microvasculature and testing of nanocarrier transport
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
Authors
The blood-brain barrier (BBB) protects the brain from pathogens but also hinders drug delivery to the central nervous system. Most of the BBB models developed up to date failed to reproduce the human anatomical complexity of brain barriers, contributing to less predictive experimental platforms and poor patient outcomes. To overcome those limitations, the development of reliable in vitro models represents a crucial step towards more effective therapies. This contribution was focused on the development of an in vitro microfluidic model of the BBB able to replicate the human neurovascular organization. The microfluidic model included human induced pluripotent stem cell-derived endothelial cells, brain pericytes, and astrocytes as self-assembled microvascular networks in a 3-dimensional fibrin gel. As previously demonstrated, the BBB model exhibited perfusable and selective microvasculature, with permeability lower than conventional in vitro models and comparable with in vivo rat brain. Permeability of polystyrene nanoparticles (NPs) and synthesized polyurethane NP was measured across the BBB model as compared to conventional Transwell assays. This physiologically relevant BBB model offers an innovative and valuable platform to preclinically predict transport efficacy of drugs and carriers.
How to Cite
PAGEPress has chosen to apply the Creative Commons Attribution NonCommercial 4.0 International License (CC BY-NC 4.0) to all manuscripts to be published.
Most read articles by the same author(s)
- Ayesha Idrees, PatrÃcia Varela, Francesca Ruini, Jeddah Marie Vasquez, Jochen Salber, Udo Greiser, Wenxin Wang, Sean McMahon, Susanna Sartori, Gianluca Ciardelli, Valeria Chiono, Drug-free antibacterial polymers for biomedical applications , Biomedical Science and Engineering: Vol. 1 No. 1 (2020)
- Valeria Chiono, Biomedical Science and Engineering: a new multidisciplinary journal dealing with modern regenerative medicine strategies , Biomedical Science and Engineering: Vol. 1 No. 1 (2020)
- Irene Carmagnola, Tiziana Nardo, Francesca Boccafoschi, Valeria Chiono, Layer-by-layer coating of stainless steel plates mediated by surface priming treatment to improve antithrombogenic properties , Biomedical Science and Engineering: Vol. 1 No. 1 (2020)
- Alice Zoso, Irene Carmagnola, Gerardina Ruocco, Mattia Spedicati, Valeria Chiono, In vitro models of human cardiac fibrotic tissue on bioartificial scaffolds , Biomedical Science and Engineering: Vol. 1 No. 1 (2020)
- Valeria Chiono, Jochen Salber, Drug-Free Antibacterial Technology for Medical Applications - First Cambridge International Conference - December 14th, 2018 - Cambridge, UK , Biomedical Science and Engineering: Vol. 1 No. 1 (2020)
- Valeria Chiono, Approaching 3R teaching in biomedical engineering , Biomedical Science and Engineering: Vol. 1 No. 1 (2020)
Similar Articles
- Emanuele Gasparotti, Margherita Cioffi, Vincenzo Positano, Emanuele Vignali, Benigno Marco Fanni, Katia Capellini, Dorela Haxhiademi, Emiliano Costa, Sergio Berti, Luigi Landini, Simona Celi, Laura Gribaldo, Analysis of non-animal methods and models for research in cardiovascular disease , Biomedical Science and Engineering: Vol. 1 No. 1 (2020)
- Paola Petrini, Daniela Pacheco, Francesco Briatico Vangosa, Cosmin S. Butnarasu, Livia Visai, Orietta Monticelli, Laura Pastorino, Sonja Visentin, Hydrogel-based platforms to mimic in vivo drug diffusion: A multicenter research , Biomedical Science and Engineering: Vol. 1 No. 1 (2020)
- Federica Rusinà, Alice Ravizza, Stefano Pasquino, Mario Muto, Simulated models for testing performance of a radiofrequency ablation device for spine tumors , Biomedical Science and Engineering: Vol. 2 No. 1 (2021)
- The Editors, Innovative approaches in cosmetic testing, in compliance with European regulations , Biomedical Science and Engineering: Vol. 3 No. 1 (2022)
- Alessandra Aldieri, Mara Terzini, Diana Massai, Cristina Bignardi, Alberto Audenino, Development of an animal-free methodology for mechanical performance assessment of engineered skin substitutes , Biomedical Science and Engineering: Vol. 1 No. 1 (2020)
- Simona Villata, Lucia Napione, Désirée Baruffaldi, Christine Nardini, Francesca Frascella, Fabrizio Pirri, Electrostimulation of a 3D in vitro skin model to activate wound healing , Biomedical Science and Engineering: Vol. 2 No. 1 (2021)
- Francesco Nevelli, Renewing potency assays: Moving forward from traditional methods , Biomedical Science and Engineering: Vol. 1 No. 1 (2020)
- Giuseppe Guagliano, Manuela Legnardim, Cristina Volpini, Nora Bloise, Silvia Dotti, Riccardo Villa, Livia Visai, Paola Petrini, Drug-induced hepatotoxicity studied in a 3D, in vitro model of the liver , Biomedical Science and Engineering: Vol. 2 No. 1 (2021)
- Salvatore Simmini, Allen C. Eaves, Sharon A. Louis, Wing Chang, Human pluripotent stem cell-derived organoids and their potential to reduce the use of animal models in research , Biomedical Science and Engineering: Vol. 3 No. 1 (2022)
- Gianfranco Beniamino Fiore, Simona Seminati, Lorenzo Pietro Coppadoro, Elia Pederzani, Marco Piola, Monica Soncini, Advanced culture systems for ex vivo human vascular tissue conditioning , Biomedical Science and Engineering: Vol. 1 No. 1 (2020)
<< < 1 2 3 4 5 6 7 8 9 10 > >>
You may also start an advanced similarity search for this article.
