Original Articles
14 February 2020
Vol. 1 No. 1 (2020)
https://doi.org/10.4081/bse.113

Clarifying mid-brain organoids: Application of the CLARITY protocol to unperfusable samples

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Brain organoids, delipidation methods, CLARITY, 3D imaging, neuron morphology
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Authors

Chiara Magliaro
Chiara.magliaro@centro3r.it
Research Center “E. Piaggio”, University of Pisa, Pisa, Italy.
Arti Ahluwalia
Research Center “E. Piaggio”, University of Pisa, Pisa; Department of Information Engineering, University of Pisa, Pisa, Italy.

The aim of this study was to apply a workflow, integrating delipidation methods and advanced 3D imaging techniques for mapping of the global neuronal organization of brain organoids. These are self-organizing constructs in vitro generated from human pluripotent stem cells encased in a Matrigel shell, which resemble downscaled structural and functional features of human brains. In particular, we focused on midbrain organoids, widely considered a promising tool for studying dopaminergic neuron degeneration in Parkinson’s Disease. The evaluation of the microanatomical alterations at a patient-level will potentially guide future research of this neuropathy, providing meaningful human specific data in line with the European Directives and the 3Rs principles.

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Clarifying mid-brain organoids: Application of the CLARITY protocol to unperfusable samples. (2020). Biomedical Science and Engineering, 1(1). https://doi.org/10.4081/bse.113

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