The 3Rs: Reduction and refinement through a multivariate statistical analysis approach in a behavioural study to unveil anxiolytic effects of natural extracts of Tilia tomentosa

  • Guendalina Olivero Department of Pharmacy (DIFAR), University of Genoa, Genoa, Italy.
  • Federica Turrini Department of Pharmacy (DIFAR), University of Genoa, Genoa, Italy.
  • Matteo Vergassola Department of Pharmacy (DIFAR), University of Genoa, Genoa, Italy.
  • Raffaella Boggia Department of Pharmacy (DIFAR), University of Genoa, Genoa, Italy.
  • Paola Zunin Department of Pharmacy (DIFAR), University of Genoa, Genoa, Italy.
  • Dario Donno Department of Agriculture, Forestry and Food Science, University of Torino, Turin, Italy.
  • Gabriele Loris Beccaro Department of Agriculture, Forestry and Food Science, University of Torino, Turin, Italy.
  • Massimo Grilli Department of Pharmacy (DIFAR), University of Genoa, Genoa; Inter-University Center for the Promotion of the 3Rs Principles in Teaching and Research, Italy.
  • Anna Pittaluga | pittalug@difar.unige.it Department of Pharmacy (DIFAR), University of Genoa, Genoa; Inter-University Center for the Promotion of the 3Rs Principles in Teaching and Research, Italy.

Abstract

We propose a multivariate statistical approach based on Principal Component Analysis (PCA) as an useful instrument to improve the Rules of Refinement and Reduction in in vivo animal experimentation. We analysed with PCA the preliminary data from a study on the effects of the oral administration of Tilia tomentosa bud extracts (TTBEs) on the behavioural skills of adult and aged male and female mice. PCA allows to rationalize the data set information and to dissect the results, showing connections among variables under study (behavioural parameters) and different trends in the experimental groups (control and TTBEs-administered animals). Our results show that PCA can give some important information that can be useful for the refinement of the experimental protocol, in order to reduce the number of the animals used in the experiments and/or the behavioural tests to get reliable information.

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Published
2020-02-14
Keywords:
Mice, refining, reducing
Statistics
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How to Cite
Olivero, G., Turrini, F., Vergassola, M., Boggia, R., Zunin, P., Donno, D., Beccaro, G. L., Grilli, M., & Pittaluga, A. (2020). The 3Rs: Reduction and refinement through a multivariate statistical analysis approach in a behavioural study to unveil anxiolytic effects of natural extracts of Tilia tomentosa. Biomedical Science and Engineering, 3(s3). https://doi.org/10.4081/bse.2019.116