Quantification of the foreign body reaction by means of a miniaturized imaging window for intravital nonlinear microscopy

  • Claudio Conci | claudio.conci@polimi.it Department of Chemistry, Materials and Chemical Engineering Giulio Natta, Politecnico di Milano, Milan, Italy.
  • Emanuela Jacchetti Department of Chemistry, Materials and Chemical Engineering Giulio Natta, Politecnico di Milano, Milan, Italy.
  • Tommaso Zandrini Department of Chemistry, Materials and Chemical Engineering Giulio Natta, Politecnico di Milano, Milan, Italy.
  • Laura Sironi Department of Physics, University of Milano-Bicocca, Milan, Italy.
  • Maddalena Collini Department of Physics, University of Milano-Bicocca, Milan, Italy.
  • Giuseppe Chirico Department of Physics, University of Milano-Bicocca, Milan, Italy.
  • Giulio Cerullo Istituto di Fotonica e Nanotecnologie (IFN)-CNR and Department of Physics, Politecnico di Milano, Milan, Italy.
  • Roberto Osellame Istituto di Fotonica e Nanotecnologie (IFN)-CNR and Department of Physics, Politecnico di Milano, Milan, Italy.
  • Manuela Teresa Raimondi Department of Chemistry, Materials and Chemical Engineering Giulio Natta, Politecnico di Milano, Milan, Italy.

Abstract

Brand new biomaterials, intended to be used on humans, must undergo in vivo quantification standardized, expensive and unethical procedures mainly based on histopathological analysis, from dissections, as defined by the ISO 10993 normative set. The aim is to prove the biomaterials biocompatibility. There exist no methods based on intravital microscopy able to satisfy the normative quantification requirements both reducing the number of employed animals and related costs. We developed a miniaturized imaging window, the Microatlas, which allows subcutaneous repeated observations in vivo of the foreign body reactions, for example to the implantation of a biomaterial. Confocal and twophoton microscopy inspections at Microatlas implantation sites demonstrated growth of the recipient tissue inside the microgrids both with micro vascularization formation and collagen generation. In conclusion, the Microatlas guided in vivo a quantifiable localized reaction inside its microscaffold, both in terms of cell repopulation, collagen and capillary formation as a probable foreign body reaction.

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Published
2020-02-14
Keywords:
Imaging window, nonlinear microscopy, two-photon polymerization
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How to Cite
Conci, C., Jacchetti, E., Zandrini, T., Sironi, L., Collini, M., Chirico, G., Cerullo, G., Osellame, R., & Raimondi, M. T. (2020). Quantification of the foreign body reaction by means of a miniaturized imaging window for intravital nonlinear microscopy. Biomedical Science and Engineering, 3(s3). https://doi.org/10.4081/bse.2019.106