Injectable thermosensitive gels for the localized and controlled delivery of biomolecules in tissue engineering/regenerative medicine

  • Monica Boffito Institute for Chemical-physical Processes, National Research Council (CNR-IPCF), Pisa; Department of Mechanical and Aerospace Engineering, Politecnico di Torino, Turin, Italy.
  • Arianna Grivet Brancot Institute for Chemical-physical Processes, National Research Council (CNR-IPCF), Pisa, Italy.
  • Ornella Lima Department of Mechanical and Aerospace Engineering, Politecnico di Torino, Turin, Italy.
  • Simona Bronco Institute for Chemical-physical Processes, National Research Council (CNR-IPCF), Pisa, Italy.
  • Susanna Sartori Department of Mechanical and Aerospace Engineering, Politecnico di Torino, Turin, Italy.
  • Gianluca Ciardelli | gianluca.ciardelli@polito.it Institute for Chemical-physical Processes, National Research Council (CNR-IPCF), Pisa; Department of Mechanical and Aerospace Engineering, Politecnico di Torino, Turin, Italy.

Abstract

The characteristic poor stability and high fluid permeability of Poloxamer®- based gels have severely limited their biomedical application. In this work, Poloxamer 407 was used as building block to synthesize a poly(ether urethane) (PEU), which aqueous solutions formed gels with improved stability and mechanics compared to Poloxamer itself. PEU chains formed micelles in aqueous solution (diameter ~40 nm at 25°C) and systems with PEU content higher than 5±1% w/v underwent a temperature- driven gelation. Gel properties were tuned acting on PEU concentration in the starting solutions, with compositions within the range 8-18% w/v showing high potential for biomedical applications (gelation at 37°C within 3-10 minutes, residence time from few days to many weeks, injectability). Model proteins (bovine serum albumin, horseradish peroxidase) were encapsulated in mild conditions and their release was modulated by gel composition (on day 3, approx. 85, 65 and 55% of encapsulated payload released from gels with 8, 15 and 18% w/v concentration). Released peroxidase retained approx. 30-40% of its activity up to 2 days, a key aspect for biomolecules in the drug delivery field.

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Published
2019-07-09
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Original Articles
Keywords:
Thermosensitive gels, Payload release, Amphiphilic poly(urethane), Model proteins
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How to Cite
Boffito, M., Grivet Brancot, A., Lima, O., Bronco, S., Sartori, S., & Ciardelli, G. (2019). Injectable thermosensitive gels for the localized and controlled delivery of biomolecules in tissue engineering/regenerative medicine. Biomedical Science and Engineering, 3(1). https://doi.org/10.4081/bse.2019.67

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