Reconstituted epithelial tissues and native cornea: A comparison of the influence of surfactants on ocular permeability


https://doi.org/10.4081/bse.123
Vol. 1 No. 1 (2020)
Published: 14 February 2020
Rabbit Cornea, reconstituted epithelial tissues, rabbit corneal epithelial cells, permeation studies, RCE
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Authors

  • Erica Zucchetti
    erica.zucchetti@phd.unipi.it
    Department of Pharmacy, University of Pisa, Pisa, Italy.
  • Daniela Monti Department of Pharmacy, University of Pisa, Pisa; Italian Inter-University Centre for the promotion of the 3Rs in teaching and research, Italy.
  • Patrizia Chetoni Department of Pharmacy, University of Pisa, Pisa; Italian Inter-University Centre for the promotion of the 3Rs in teaching and research, Italy.
  • Silvia Tampucci Department of Pharmacy, University of Pisa, Pisa; Italian Inter-University Centre for the promotion of the 3Rs in teaching and research, Italy.
  • Susi Burgalassi Department of Pharmacy, University of Pisa, Pisa; Italian Inter-University Centre for the promotion of the 3Rs in teaching and research, Italy.

The aim of this study was to prepare an artificial rabbit corneal epithelium (RRCE) to compare with a human corneal epithelial model and excised rabbit cornea through permeation studies to investigate differences of surfactants influence on ocular permeability of a lipophilic compound. First solubility assays with different surfactants were performed and the integrity of the RRCE was assessed by measuring transepithelial electrical resistance (TEER). The permeation parameters showed that the RRCE was more sensitive than native cornea and human cornal epithelial model to the effect of permeation enhancers.


Zucchetti, E., Monti, D., Chetoni, P., Tampucci, S., & Burgalassi, S. (2020). Reconstituted epithelial tissues and native cornea: A comparison of the influence of surfactants on ocular permeability. Biomedical Science and Engineering, 1(1). https://doi.org/10.4081/bse.123

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