Clarifying mid-brain organoids: Application of the CLARITY protocol to unperfusable samples

  • Chiara Magliaro | Chiara.magliaro@centro3r.it Research Center “E. Piaggio”, University of Pisa, Pisa, Italy.
  • Arti Ahluwalia Research Center “E. Piaggio”, University of Pisa, Pisa; Department of Information Engineering, University of Pisa, Pisa, Italy.

Abstract

The aim of this study was to apply a workflow, integrating delipidation methods and advanced 3D imaging techniques for mapping of the global neuronal organization of brain organoids. These are self-organizing constructs in vitro generated from human pluripotent stem cells encased in a Matrigel shell, which resemble downscaled structural and functional features of human brains. In particular, we focused on midbrain organoids, widely considered a promising tool for studying dopaminergic neuron degeneration in Parkinson’s Disease. The evaluation of the microanatomical alterations at a patient-level will potentially guide future research of this neuropathy, providing meaningful human specific data in line with the European Directives and the 3Rs principles.

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Published
2020-02-14
Keywords:
Brain organoids, delipidation methods, CLARITY, 3D imaging, neuron morphology
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How to Cite
Magliaro, C., & Ahluwalia, A. (2020). Clarifying mid-brain organoids: Application of the CLARITY protocol to unperfusable samples. Biomedical Science and Engineering, 3(s3). https://doi.org/10.4081/bse.2019.113