Use of an in vitro model of hepatic steatosis for studying the anti-oxidant and antisteatotic effects of fucoidan polysaccharides

  • Zeinab El Rashed Department of Earth, Environmental and Life Sciences, University of Genoa, Genoa, Italy.
  • Hala Khalife Rammal Rammal Laboratory (ATAC group), Faculty of Sciences l, Lebanese University, Lebanon.
  • Adriana Voci Department of Earth, Environmental and Life Sciences, University of Genoa, Genoa, Italy.
  • Elena Grasselli Department of Earth, Environmental and Life Sciences, University of Genoa, Genoa, Italy.
  • Laura Canesi Department of Earth, Environmental and Life Sciences, University of Genoa, Genoa, Italy.
  • Ilaria Demori | idemori@unige.it Department of Earth, Environmental and Life Sciences, University of Genoa, Genoa, Italy.

Abstract

Non Alcoholic Fatty Liver Disease (NAFLD) is characterised by fat accumulation in hepatocytes in the form of triacyglycerols (TAGs) within cytosolic lipid droplets. Fucoidans (FUs) are biologically active polysaccharides usually isolated from brown marine algae, but recently identified also in terrestrial plants. In this study, we aimed to investigate the anti-oxidant and anti-steatotic effects of FUs purified from C. compressa, F. hermonis, and E. globulus. To this aim, we used a validated NAFLD in vitro model consisting of rat hepatoma FaO cells exposed to an oleate/palmitate mixture. Such a model is suitable for rapid investigation of direct effects of natural and artificial compounds, together with satisfying the strategy of 3Rs for laboratory use of animals. Our results indicated that all FUs display anti-oxidant and anti-steatotic activities. Steatotic FaO cells may be employed to further study the biological effects of FUs.

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Published
2020-02-14
Keywords:
Non alcoholic fatty liver disease, fucoidan, anti-steatotic, anti-oxidant
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How to Cite
El Rashed, Z., Khalife, H., Voci, A., Grasselli, E., Canesi, L., & Demori, I. (2020). Use of an in vitro model of hepatic steatosis for studying the anti-oxidant and antisteatotic effects of fucoidan polysaccharides. Biomedical Science and Engineering, 3(s3). https://doi.org/10.4081/bse.2019.109