Ink-jet printed chitosan precise patterning for engineered 2D neuronal networks


https://doi.org/10.4081/bse.148
Vol. 2 No. 1 (2021)
Published: 29 September 2021
Chitosan, ink-jet printing, patterned neuronal network, microelectrode arrays (MEAs)
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Authors

  • Andrea Andolfi University of Genoa, Genoa, Italy , Italy.
  • Pietro Arnaldi Department of Informatics, Bioengineering, Robotics and System Engineering, University of Genova, Genova, Italy.
  • Donatella Di Lisa Department of Informatics, Bioengineering, Robotics and System Engineering, University of Genova, Genova, Italy.
  • Sergio Martinoia Department of Informatics, Bioengineering, Robotics and System Engineering, University of Genova, Genova, Italy.
  • Laura Pastorino
    laura.pastorino@unige.it
    Department of Informatics, Bioengineering, Robotics and System Engineering, University of Genova, Genova, Italy.

The aim of this work was to demonstrate chitosan micropatterning effectiveness to realize spatially guided 2D neuronal networks of primary rat neurons and neurons derived from human induced pluripotent stem cells (h-iNeurons). Morphological characterizations were carried out to validate the formation of a well-defined neuronal networks.


Andolfi, A., Arnaldi, P., Di Lisa, D. ., Martinoia, S., & Pastorino, L. (2021). Ink-jet printed chitosan precise patterning for engineered 2D neuronal networks. Biomedical Science and Engineering, 2(1). https://doi.org/10.4081/bse.148

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